SMAP v -1 - A primer design program specificially for SMAP
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Primer Design Form Page
SmartAmp™ Primer Set Image
Paste your sequence here:
TCTTGCCCCATGGCCGGACCTCCCCACCCTGGCGCCCAATACCGCCAACACCAGTGGGCTGCCAGGGGTTCCGTGGGAGGCGGCCCTAGCCGGGGCCCTGCTGGCGCTGGCGGTGCTGGCCACCGTGGGAGGCAACCTGCTGGTCATCGTGGCCATCGCCTGGACTCCGAGACTCCAGACCATGACCAACGTGTTCGTGACTTCGCTGGCCGCAGCCGACCTGGTGATGGGACTCNTGGTGGTGCCGCCGGCGGCCACCTTGGCGCTGACTGGCCACTGGCCGTTGGGCGCCACTGGCTG
SNP position in sequence:
SNP base in sequence:
A
C
G
T
Primer on which to locate SNP:
TP-region Bc
BP
FP
TP-region A
Sequence is from:
NONE
HUMAN
RODENT
Primer Options:
Option for Primer temperature
Minimum
Maximum
TP-region A / FP
40
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°C
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°C
TP-region Bc
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°C
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°C
BP
40
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°C
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65
°C
OP1 / OP2
40
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°C
40
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65
°C
options for distances between primers
Minimum
Maximum
OP1 … TP-region A
0
1
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bp
0
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40
bp
FP … OP2
0
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bp
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bp
TP-region Bc … FP
0
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bp
0
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bp
Copyright © 2006 RIKEN, Japan.
All rights reserved.
Designed by: Alistair Chalk and
NTT Software Corporation.